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kcnd2  (Alomone Labs)


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    Alomone Labs kcnd2
    Kcnd2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kcnd2/pm41819103-1093-45-46?v=Alomone+Labs
    Average 94 stars, based on 3 article reviews
    kcnd2 - by Bioz Stars, 2026-07
    94/100 stars

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    Figure 6. Validation of biomarkers. (A) The mRNA expression of KCND2 and VSTM4 in 20 pairs of tissues, including TNBC adjacent cancer and tumor tissues. (B) The protein expression of KCND2 was verified in cell lines. (C) The expression of KCND2 in the TNBC adjacent cancer and tumor tissues was verified by immunohistochemistry. NS, no significance; ***P < 0.001.

    Journal: Aging

    Article Title: Constructing a novel prognostic model for triple-negative breast cancer based on genes associated with vasculogenic mimicry.

    doi: 10.18632/aging.205806

    Figure Lengend Snippet: Figure 6. Validation of biomarkers. (A) The mRNA expression of KCND2 and VSTM4 in 20 pairs of tissues, including TNBC adjacent cancer and tumor tissues. (B) The protein expression of KCND2 was verified in cell lines. (C) The expression of KCND2 in the TNBC adjacent cancer and tumor tissues was verified by immunohistochemistry. NS, no significance; ***P < 0.001.

    Article Snippet: Endogenous peroxidase activity was eliminated using 3% H2O2, and the sections were incubated with KCND2 (21298-1-AP, Proteintech) at 4° C for an overnight duration.

    Techniques: Biomarker Discovery, Expressing, Immunohistochemistry

    Figure 7. Depletion of KCND2 and VSTM4 reduces the proliferation and migration of TNBC cells in vitro. (A) The knockdown efficiency of siRNA targeting KCND2 in BT-549 cells was verified. (B) EdU incorporation analysis and (C) CCK-8 assay revealed that the knockdown of KCND2 affected the cell proliferation in BT-549 cells. (D) The knockdown efficiency of siRNA targeting VSTM4 in MDA-MB-231 cells was verified. (E) EdU incorporation analysis and (F) CCK-8 assay revealed that the knockdown of VSTM4 impacts cell proliferation in MDA-MB-231 cells. (G) The wound healing assay demonstrated that the knockdown of KCND2 affected the migration of BT-549 cells. (H) Wound healing assay results showed that the knockdown of VSTM4 impacted the migration of MDA-MB-231 cells. *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Aging

    Article Title: Constructing a novel prognostic model for triple-negative breast cancer based on genes associated with vasculogenic mimicry.

    doi: 10.18632/aging.205806

    Figure Lengend Snippet: Figure 7. Depletion of KCND2 and VSTM4 reduces the proliferation and migration of TNBC cells in vitro. (A) The knockdown efficiency of siRNA targeting KCND2 in BT-549 cells was verified. (B) EdU incorporation analysis and (C) CCK-8 assay revealed that the knockdown of KCND2 affected the cell proliferation in BT-549 cells. (D) The knockdown efficiency of siRNA targeting VSTM4 in MDA-MB-231 cells was verified. (E) EdU incorporation analysis and (F) CCK-8 assay revealed that the knockdown of VSTM4 impacts cell proliferation in MDA-MB-231 cells. (G) The wound healing assay demonstrated that the knockdown of KCND2 affected the migration of BT-549 cells. (H) Wound healing assay results showed that the knockdown of VSTM4 impacted the migration of MDA-MB-231 cells. *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: Endogenous peroxidase activity was eliminated using 3% H2O2, and the sections were incubated with KCND2 (21298-1-AP, Proteintech) at 4° C for an overnight duration.

    Techniques: Migration, In Vitro, Knockdown, CCK-8 Assay, Wound Healing Assay

    Figure 6. Validation of biomarkers. (A) The mRNA expression of KCND2 and VSTM4 in 20 pairs of tissues, including TNBC adjacent cancer and tumor tissues. (B) The protein expression of KCND2 was verified in cell lines. (C) The expression of KCND2 in the TNBC adjacent cancer and tumor tissues was verified by immunohistochemistry. NS, no significance; ***P < 0.001.

    Journal: Aging

    Article Title: Constructing a novel prognostic model for triple-negative breast cancer based on genes associated with vasculogenic mimicry.

    doi: 10.18632/aging.205806

    Figure Lengend Snippet: Figure 6. Validation of biomarkers. (A) The mRNA expression of KCND2 and VSTM4 in 20 pairs of tissues, including TNBC adjacent cancer and tumor tissues. (B) The protein expression of KCND2 was verified in cell lines. (C) The expression of KCND2 in the TNBC adjacent cancer and tumor tissues was verified by immunohistochemistry. NS, no significance; ***P < 0.001.

    Article Snippet: Antibodies to KCND2 (21298-1-AP), were purchased from Proteintech (Wuhan, China).

    Techniques: Biomarker Discovery, Expressing, Immunohistochemistry

    Figure 7. Depletion of KCND2 and VSTM4 reduces the proliferation and migration of TNBC cells in vitro. (A) The knockdown efficiency of siRNA targeting KCND2 in BT-549 cells was verified. (B) EdU incorporation analysis and (C) CCK-8 assay revealed that the knockdown of KCND2 affected the cell proliferation in BT-549 cells. (D) The knockdown efficiency of siRNA targeting VSTM4 in MDA-MB-231 cells was verified. (E) EdU incorporation analysis and (F) CCK-8 assay revealed that the knockdown of VSTM4 impacts cell proliferation in MDA-MB-231 cells. (G) The wound healing assay demonstrated that the knockdown of KCND2 affected the migration of BT-549 cells. (H) Wound healing assay results showed that the knockdown of VSTM4 impacted the migration of MDA-MB-231 cells. *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Aging

    Article Title: Constructing a novel prognostic model for triple-negative breast cancer based on genes associated with vasculogenic mimicry.

    doi: 10.18632/aging.205806

    Figure Lengend Snippet: Figure 7. Depletion of KCND2 and VSTM4 reduces the proliferation and migration of TNBC cells in vitro. (A) The knockdown efficiency of siRNA targeting KCND2 in BT-549 cells was verified. (B) EdU incorporation analysis and (C) CCK-8 assay revealed that the knockdown of KCND2 affected the cell proliferation in BT-549 cells. (D) The knockdown efficiency of siRNA targeting VSTM4 in MDA-MB-231 cells was verified. (E) EdU incorporation analysis and (F) CCK-8 assay revealed that the knockdown of VSTM4 impacts cell proliferation in MDA-MB-231 cells. (G) The wound healing assay demonstrated that the knockdown of KCND2 affected the migration of BT-549 cells. (H) Wound healing assay results showed that the knockdown of VSTM4 impacted the migration of MDA-MB-231 cells. *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: Antibodies to KCND2 (21298-1-AP), were purchased from Proteintech (Wuhan, China).

    Techniques: Migration, In Vitro, Knockdown, CCK-8 Assay, Wound Healing Assay